Video Transcript
We want to know the genetic tools that's not appropriately paired with its correct use. So a is Dina Pullem rice, which Ah, it's match with amplifying DNA using PCR, which is correct. DNA pull. Emery's obviously goes and makes new strands of DNA based on a template, so this one is going to be true. But again, we're looking for which one is not appropriately a paired. So since it's true, this is not going to be your answer. Reverse transcriptase. Ah, they ask if it's used for creating a CD and a library from Arne so seedy and libraries basically all the genes they're expressed. So if you reverse transcribe R N a using reverse transcriptase, you could get a CD and a library. So this is true, and therefore it will again, not the are correct answer, because we're looking for what's not appropriately paired CD and a lie Gays. They suggest that it might be involved in cleaving DNA, producing three prime or five prime overhanging specific locations. So this eyes not going to be true. So Dina Lie Gaze is basically used to join DNA together, its restriction enzymes that cleave DNA and can produce the three prime or five prime ends in certain locations otherwise known a sticky ends. So this one is going to be the correct answer because it's incorrect. It's inappropriately paired and then electro freezes separating. And I don't find DNA fragments based on size. That's a perfectly reasonable use for electro free sis. So again, this is not going to be our answer. Our answer. You seek.
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Solution
The correct option is B DNA ligase - multiplication of rDNA molecules
DNA ligase is an enzyme which joins or seals the gaps between DNA fragments by formation of phosphodiester bonds. It has no role to play in the multiplication of rDNA molecules.
EcoR I is a restriction endonuclease that recognises a specific palindromic sequence and makes cuts at these specific locations to form sticky ends in the DNA. The ends with single-stranded DNA “overhangs” are called sticky ends.
DNA polymerase enzyme helps in the synthesis of a DNA strand by adding deoxyribonucleotides to the 3' end of a primer. It is used in polymerase chain reactions [PCR] for amplifying the DNA segments.
Selectable markers are genes that help in identification and selection of transformants over non-transformants.
Transformants are those cells that have undergone genetic transformation through the uptake of foreign DNA.
Non-transformants
are those cells which have not taken up the foreign DNA.
Generally, genes that confer resistance to organisms against antibiotics, serve as selectable markers.
EcoRl -Production of sticky ends DNA ligase - Multiplication of DNA molecules ori- copy number Selectable market - Identification of transformants
Answer : B
Solution : `DNA` ligases are also called genetic gum. They join two indiviual fragments of double stranded DNA by forming phosphodiester bonds between them thus help in sealing of DNA fragments. Therefore acts as molecular glue. The enzyme used most often is `T_[4]` DNA ligase.
EcoRl -Production of sticky ends DNA ligase - Multiplication of DNA molecules ori- copy number Selectable market - Identification of transformants
Answer : B
Solution : `DNA` ligases are also called genetic gum. They join two indiviual fragments of double stranded DNA by forming phosphodiester bonds between them thus help in sealing of DNA fragments. Therefore acts as molecular glue. The enzyme used most often is `T_[4]` DNA ligase.