Which of the following items are needed to perform a venipuncture using the vacutainer method?
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You are here: Geisinger Medical Laboratories > Specimen Collection Manual and Test Catalog > Blood Specimen Collection and ProcessingThe first step in acquiring a quality lab test result for any patient is the specimen collection procedure. The venipuncture procedure is complex, requiring both knowledge and skill to perform. Several essential steps are required for every successful collection procedure: Venipuncture Procedure:
Fingerstick Procedure:
Heelstick Procedure (infants):The recommended location for blood collection on a newborn baby or infant is the heel. The diagram below indicates the proper area to use for heel punctures for blood collection.
Order of Draw:Blood collection tubes must be drawn in a specific order to avoid cross-contamination of additives between tubes. The recommended order of draw is:
Labeling The SampleAll specimens must be received by the laboratory with a legible label containing at least two (2) unique identifiers. The specimen must be labeled with the patient's full name (preferably last name first, then first name last) and one of the following:
Areas to Avoid When Choosing a Site for Blood Draw:Certain areas are to be avoided when choosing a site for blood draw:
Techniques to Prevent Hemolysis (which can interfere with many tests):
Blood Sample Handling and Processing:Pre-centrifugation Handling - The first critical step in the lab testing process, after obtaining the sample, is the preparation of the blood samples. Specimen integrity can be maintained by following some basic handling processes:
What are the materials needed for venipuncture?At a minimum, the equipment required when drawing blood includes gloves, alcohol or iodine to cleanse the area, a tourniquet, tubes, a tube holder, needles, tape, and gauze. A Phlebotomist should be completely aware of the wide range of needles, tubes, etc.
Which of the following materials are needed for a capillary puncture?Capillary punctures require different devices than the typical venipuncture equipment. They include lancets, microcontainer tubes, microhematocrit tubes and sealants, and warming devices.
When drawing blood by the vacutainer method it is important to fill the tubes in the recommended order which one of the following tubes should be filled first?This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.
Which of the following vacutainer tubes is routinely used for hematology testing?a. Purple top – K2 or K3 EDTA tube used for most hematology studies.
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